Table 1 Primer sequences for RT-qPCR
From: MicroRNA-223-3p downregulates the inflammatory response in preeclampsia placenta via targeting NLRP3
Gene name | Sequence | |
|---|---|---|
Forward (5’-3’) | Reverse (5’-3’) | |
miR-223-3p | TGTCAGTTTGTCAAATACCCCA | universal primer |
NLRP3 | GAGGAAAAGGAAGGCCGACA | TGGCTGTTCACCAATCCATGA |
ASC | TCACCGCTAACGTGCTG | TGGTCTATAAAGTGCAGGCC |
Caspase-1 | TTTCTGCTCTTCCACACCAG | AATGAAAATCGAACCTTGCGG |
GSDMD | TGGTCTATAAAGTGCAGGCC | GACGTCCAAGTCAGAGTCAATAA |
IL-1β | GGACAGGATATGGAGCAACAA | CCCAAGGCCACAGGTATTT |
IL-18 | CCAAGGAAATCGGCCTCTATT | CATACCTCTAGGCTGGCTATCT |
U6 | universal primer | universal primer |
GAPDH | CGTGGAAGGACTCATGACCA | GGCAGGGATGATGTTCTGGA |