Fig. 4

Effect of miR-223-3p on LPS-induced NLRP3 inflammasome mediated signaling pathway in HTR8/SVneo cells. (A) The HTR8/SVneo cells were transfected with miR-223-3p overexpression plasmids/miR-223-3p negative control plasmids for 48 h, then induced with LPS (500 ng/ml) for 24 h. RT-qPCR was performed to determine the expression level of miR-223-3p. (B) The expression of NLRP3, Caspase-1, GSDMD, IL-1β, and IL-18 mRNA in HTR8/SVneo cells with above treatment was detected by RT-qPCR. (C) The expression of IL-1β and IL-18 in the culture supernatant of HTR8/SVneo cells was assessed by ELISA. All data were expressed as mean ± SD; The data were analyzed by Two-way ANOVA (Tukey’s multiple comparisons test), *P < 0.05; ** P Δ 0.01; *** P Δ 0.001 vs. the blank group, # P Δ 0.05; ## P Δ 0.01; ### P Δ 0.001 vs. the LPS group; △P Δ 0.05; △△P Δ 0.01; △△△P Δ 0.001 vs. the miR-223-3p + LPS group, the experiments were independently repeated three times. miR-223-3p, microRNA-223-3p; NLRP3, Nod-like receptor pyrin domain-containing 3; Caspase-1, the effector protein precysteine hydrolase-1; GSDMD, gasdermin D; IL-, interleukin; LPS, lipopolysaccharide; RT-qPCR, real time quantitative polymerase chain reaction; ELISA, Enzyme-linked immunosorbent; SD, standard deviation