Fig. 3

Expression of NLRP3 inflammasome-related molecules in HTR8/SVneo cells exposed to lipopolysaccharide (LPS). (A) The expression of NLRP3, Caspase-1, GSDMD, IL-1β, and IL-18 mRNA in HTR8/SVneo cells treated with different concentrations of LPS for 24 h was detected by RT-qPCR. (B) The protein expression of NLRP3, Caspase-1 and GSDMD in HTR8/SVneo cells was detected by western blot. (C) The expression of IL-1β and IL-18 in the culture supernatant of HTR8/SVneo cells was assessed by ELISA. All data were expressed as mean ± SD; The data were analyzed by Two-way ANOVA (Tukey’s multiple comparisons test), * P < 0.05; **P < 0.01; ***P < 0.001 vs. the blank group, the experiments were independently repeated three times. NLRP3, Nod-like receptor pyrin domain-containing 3; Caspase-1, the effector protein precysteine hydrolase − 1; GSDMD, gasdermin D; IL-, interleukin; LPS, lipopolysaccharide; RT-qPCR, real time quantitative polymerase chain reaction; ELISA, Enzyme-linked immunosorbent; SD, standard deviation