Table 2 Designed primers for ARMS-PCR reactions and annealing temperature
SNP | Primer sequence | Annealing temperature (°C) |
|---|---|---|
rs20541 (G/A) | F: CTTCCGTGAGGACTGAATGA RT: CTTTCGAAGTTTCAGTTGAACT RC: CTTTCGAAGTTTCAGTTGAACC F-PCR-Control: CCTCTGCACAGTTTGGAC R-PCR-Control: TCTGTCCAGCAATCCAGG | 52 °C |
rs56035208 (G/C) | F: TGTCAGGCGTCACCACTT RC: GACCAGCTCCTCAATGAGC RG: GACCAGCTCCTCAATGAGG F-PCR-Control: CCTCTGCACAGTTTGGAC R-PCR-Control: TCTGTCCAGCAATCCAGG | 53 °C |
rs1028181(T/C) | F: GCAAATGTGCTCAGTACTTG RT: CGT TTA ATC GCT CCT TAC AGT RC: CGT TTA ATC ATA GCT CCT TAC AGC F-PCR-Control: ATA TGG ATG CTT CAC ACA GAC C R-PCR-Control: TTC CCT GTA GTC AGG AAG | 58.5 °C |
rs2243191(T/C) | F: ACC TCA GGG AAG ATG T RT: CCT TGT CAT CAA GCT GAC A RC: CCT TGT CAT CAA GCT GAC G F-PCR-Control: CCT CTG CAC AGT TTG GAC R-PCR-Control: TCT CAG CAA TCC AGG | 54 °C |