Evaluation of highly sensitive diagnostic tools for the detection of P. falciparum in pregnant women attending antenatal care visits in Colombia

Table 2 Plasmodium spp. positivity rate by diagnostic test in peripheral blood samples collected from pregnant women

Positive test: n (%) [N = 858]	*Plasmodium* spp.	Species-specific			Non-*P.f.*
Positive test: n (%) [N = 858]	*Plasmodium* spp.	*P. falciparum*	*P. vivax*	*P. malariae*	Non-*P.f.*
Microscopy	23 (2.7%)	23 (2.7%) ^c	1 (0.1%) ^c	0 (0%)	N/A
cRDT^a	21 (2.4%)	21 (2.4%) ^c	1 (0.1%) ^c	N/A	N/A
hsRDT^b	N/A	26 (3.0%)	N/A	N/A	N/A
LAMP	45 (5.2%)	36 (4.2%)	N/A	N/A	9 (1.0%)
nPCR	36 (4.2%)	30 (3.5%) ^c	4 (0.5%) ^c	3 (0.3%)	N/A
qRT-PCR	47 (5.5%)	39 (4.5%) ^d	N/A	N/A	9 (1.0%) ^d

cRDT conventional Rapid Diagnostic Test, hsRDT highly sensitive Rapid Diagnostic Test, nPCR nested Polymerase Chain Reaction, LAMP Loop-mediated isothermal amplification, qRT-PCR Quantitative Reverse Transcription polymerase chain reaction
^aOnly P. falciparum and P. vivax. ^bOnly P. falciparum detected. Five samples (0.6%) with indeterminate results. ^cIncludes one mixed infection of P. falciparum and P. vivax. ^dIncludes one mixed infection of P. falciparum and non-P. falciparum species

ISSN: 1471-2393