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Table 4 Data from phenotypic characterization and multiplex real-time PCR of Streptococcus agalactiae

From: Development of a melting-curve based multiplex real-time PCR assay for simultaneous detection of Streptococcus agalactiae and genes encoding resistance to macrolides and lincosamides

Isolates

Susceptibility phenotype

Real-time multiplex PCR

 

Ea

DAb

cfb c

erm(A)c

erm(B)c

mef(A/E)c

LMC UEL 5

S

S

+

–

–

–

LMC UEL 21

S

S

+

–

–

–

LMC UEL 23

S

S

+

–

+

–

LMC UEL 27

S

S

+

–

–

–

LMC UEL 30

S

S

+

–

–

–

LMC UEL 34

S

S

+

–

–

–

LMC UEL 43

S

S

+

–

–

–

LMC UEL 43A

R

R

+

+

+

–

LMC UEL 57

S

S

+

+

–

+

LMC UEL 60

S

S

+

–

–

–

LMC UEL 68

S

S

–

–

–

–

LMC UEL 103

R

S

+

–

–

+

LMC UEL 28

CN

CN

+

–

–

–

LMC UEL 63

CN

CN

+

–

+

+

LMC UEL 95

CN

CN

+

–

–

+

LMC UEL 99

CN

CN

+

–

+

+

  1. aE (erythromycin) and bDA (clindamycin) resistance phenotypes were determined by the double-disk diffusion method [34]. (S) Susceptible; (R) Resistant; (CN) Culture-Negative. cTarget genes detected in vaginal-rectal swab specimens by multiplex real-time PCR. (+) Presence; (−) Absence
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BMC Pregnancy and Childbirth

ISSN: 1471-2393

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